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|Lab-lemco meat extract (Oxid)||2.4||g|
|Proteose peptone No.3 (Difco)||10.0||g|
|Bacto yeast extract (Difco)||5.0||g|
** ph : 7.6-7.8
** memo :
** additional notes : Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6 - 7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.