KCTC Media No. 366 : Mineral Medium for Chemolithotrophic Growth (H-3)

Composition	capacity	unit
KH2PO4	2.3	g
Na2HPO4·2H2O	2.9	g
NH4Cl	1.0	g
MgSO4·7H2O	0.5	g
NaHCO3	0.5	g
CaCl2·2H2O	0.01	g
Fe(NH4) citrate	0.05	g
trace element solution SL-6 (see medium 339)	5.0	ml
Distilled water	980.0	ml
Agar (if necessary)	15.0	g

** ph : 6.8

** memo : Autoclave for 15 minutes at 121℃. Sterilize Fe(NH_4_) citrate (0.05 g in 20 ml H_2_O) separetely and then add to the medium.
For chemolithotrophic growth incubate the culture under an atmospere of 2 % (v/v) O_2_, 10 % CO_2_, 60 % H_2_ and 28 % N_2_.
For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% (w/v) carbohydate or 0.1% (v/v) organic acid). For growth on nitrogen-free medium, omit NH_4_Cl and incubate the culture under an atmosphere of 2 % (v/v) O_2_, 10 % CO_2_, 10 % H_2_ and 78 % N_2_ or heterotrophically under 2 % (v/v) O_2_ and 98 % N_2_ nitrogen. For more details see Ref. 511 and Ref.1483"

** additional notes : -