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Composition | capacity | unit |
---|---|---|
Trypticase Soy Broth (BBL no. 11774) | 5.5 | g |
Resazurin | 0.5 | mg |
Glucose | 2.0 | g |
ph
Prepare TSB anaerobically under 100% N_2_. Adjust pH to 5.5 prior to dispensing and autoclaving. Add glucose from separately sterilized, anaerobic 20% (w/v) solution.