Korean Collection for Type Cultures

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KCTC Media No. 881 : Patel lab. (PL) Medium

Composition

capacity

unit

NH₄Cl

1.0

K₂HPO₄

0.6

KH₂PO₄

0.3

MgCl₂·6H₂O

0.1

CaCl₂·2H₂O

0.1

NaCl

1.0

HEPES

12.0

Wolin's Vitamin Soution (l-1 deionized water)

Composition	capacity	unit
biotin	2.0	mg
folic acid	2.0	mg
pyridoxine-HCl	10.0	mg
riboflacin	5.0	mg
thiamine	5.0	mg
nicotinic acid	5.0	mg
pantothenic acid	5.0	mg
para-amino benzoic acid	5.0	mg
thiotic acid	5.0	mg
The solution was filter-sterilised (Millex GS 0.22um filter unit, Millipore, Ireland) and stored at 4C
vitamin B₁₂	0.1	mg

Ziekus Trace Elements

Yeast extract

0.2

sodium sulfate

2.84

1.0

After sterilisation of media glycerol was to a final concentration of 0.2% from a sterile anaerobic stock solution.

Ziekus` Trance Element Solution (Zeikus et al., 1979)

Composition	capacity	unit
NaCl	1.0	g
Nitrilotriacetic acid	12.5	g
The pH of the solution was adjusted to 6.5 and was sterilized for 30min at 121C and 1-1.5kg cm-2 pressure. The solution was stored at 4C
FeCl₃ . 4H₂O	0.2	g
MnCl₂ . 4H₂O	0.1	g
CoCl₂ . 6H₂O	0.0017	g
CaCl₂ . H₂O	0.1	g
ZnCl₂	0.1	g
CuCl₂	0.2	g
H₃BO₃	0.01	g
NaMoO₄ . 2H₂O	0.01	g
Na₂SeO₃	0.02	g

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Korean Collection for Type Cultures (KCTC)
Korea Research Institute of Bioscience and Biotechnology (KRIBB)

181 Ipsin-gil. Jeongeup-si, Jeollabuk-do 56212, Korea