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자원검색

KCTC Media No. 1535 : KCTC15839 MEDIUM

조성 용량 단위
 KH2PO4 0.33  g/L 
 NH4Cl 0.33  g/L 
 KCl 0.33  g/L 
 MgCl2·6H2O 0.33  g/L 
 CaCl2·6H2O 0.33  g/L 
 NaHCO3 2.0  g/L 
 NaOH 0.6  g/L 
 resasurin solution 0.0002 
 The vitamins (Wolin EA, Wolin MJ, Wolfe RS (1963) (See medium 1532) 1.0  ml/L 
 trace-elements (See medium 1532) 1.0  ml/L 

pH 6-6.5


NaHCO3 ,NaOH and vitamins, Na2S·9H20 (0.5 g/l) are added after boiling and cooling of the medium under 100% CO2.
Adjust the PH between 6.0 and 6.5 with sterile HCl or NaOH after autoclaving.
2g/l glucose is used as a growth substrate and added separately.
Yeast extract is obligatory for growth and at least 50mg/l YE must be added to the final solution befor inoculation.