자원검색

자원검색

KCTC Media No. 941 : KPL MEDIUM

조성 용량 단위
 Lactic acid whey (see below) 1000  ml 
 White table wine (see below) 140  ml 
 Glucose 10 
 Galactose 10 
 Tween 80 1.0  ml 
 Agar 15 
  Adjust pH to 5.5    
 Lactic acid whey: Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100°C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100°C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl.    
 White table wine: Filter-sterilize and add to autoclaved basal medium