KCTC Media No. 1108 : SI Medium
조성 |
용량 |
단위 |
Trace element sol. |
1.0 |
ml |
조성 |
용량 |
단위 |
Nitrilotriacetic acid |
12.8 |
g |
CaCl2·2H2O |
0.1 |
g |
CoCl2·6H2O |
0.024 |
g |
FeCl3·6H2O |
1.35 |
g |
MnCl2·4H2O |
0.1 |
g |
ZnCl2 |
0.1 |
g |
H3BO3 |
0.01 |
g |
NiCl2·6H2O |
0.12 |
g |
Na2MoO4·2H2O |
0.024 |
g |
CuCl2·2H2O |
0.025 |
g |
Distilled water |
1.0 |
L |
Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH. |
|
|
|
Se/W sol. |
1.0 |
ml |
조성 |
용량 |
단위 |
Na2SeO3 ·5H2O |
4 |
mg |
Na2WO4·2H2O |
4 |
mg |
Distilled water |
1.0 |
L |
|
Solution A: |
|
|
조성 |
용량 |
단위 |
Yeast extract (BD-Difco) |
0.1 |
g |
KH2PO4 |
0.14 |
g |
MgCl2.6H2O |
0.2 |
g |
CaCl2.2H2O |
0.015 |
g |
NH4Cl |
0.54 |
g |
Vitamin sol.(see medium 334) |
1.0 |
ml |
Trace element sol. |
1.0 |
ml |
Se/W sol. |
1.0 |
ml |
NaHCO3 |
2.5 |
g |
Resazurin |
1.0 |
mg |
Distilled water |
900 |
ml |
Mix components except NaHCO3 and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense 0.9 volume of the medium into culture vessels (e.g., 18 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. |
|
|
|
Solution B: |
|
|
조성 |
용량 |
단위 |
Glucose |
0.18 |
g |
Distilled water |
100 |
ml |
Adjust pH to 7.0 and filter-sterilize. Keep the solution under a N2 atmosphere |
|
|
|
** ph : -
** memo : -
** 추가조치 사항 : To complete the medium, add 0.1 volume of Solution B to the culture vessels containing Solution A. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine·HCl·H2O and Na2S·9H2O solutions to the medium (3% solutions, autoclaved and stored under a N2 atmosphere).