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조성 | 용량 | 단위 |
---|---|---|
Trypticase peptone (BD BBL) | 5.0 | g |
Peptone from meat (pepsin·digested) | 5.0 | g |
Yeast extract | 10.0 | g |
Na-Resazurin solution(0.1% w/v) | 0.5 | ml |
Salt solution (see medium 1477) | 40 | ml |
L-Cysteine·HCl ·H2O | 0.5 | g |
Na2CO3 | 1.0 | g |
D-Glucose | 5.0 | g |
D·W | 1.0 | L |
** ph : -
** memo : Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with
80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then dispense under
80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add
glucose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and carbonate
from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of
complete medium
to 7.0, if necessary.
** 추가조치 사항 : For KCTC5071 adjust pH to 6.3.
For KCTC25034 replace glucose with 5g/l gamma-aminobutyrate.
For KCTC25281 replace glucose with 10g/l maltose.