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조성 | 용량 | 단위 |
---|---|---|
Solution A: | ||
NaCl | 25 | g |
K2HPO4 | 0.5 | g |
NH4Cl | 1.0 | g |
Na2SO4 | 1.0 | g |
CaCl2·2H2O | 0.1 | g |
MgSO4·7H2O | 2.0 | g |
Na-DL-lactate | 2.0 | g |
Yeast extract | 1.0 | g |
Na-resazurin solution (0.1% w/v) | 0.5 | ml |
Distilled water | 980 | ml |
Solution B: | ||
FeSO4·7H2O | 0.5 | g |
Distilled water | 10.0 | ml |
Solution C: | ||
Na-thioglycolate | 0.1 | g |
Ascorbic acid | 0.1 | g |
Distilled water | 10.0 | ml |
** ph : 7.0
** memo : Bring solution A to the boil, then cool to room temperature while sparging with 100% N2
gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas
atmosphere into anoxic Hungate-type tubes. During distribution continuously swirl the
medium to keep the grey precipitate suspended. Autoclave 15 min at 121?C. Adjust pH
of the complete medium to 6.8 - 7.0, if necessary.
For DSM 10520 adjust final pH of autoclaved medium to 7.5 using a sterile anoxic stock
solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.
** 추가조치 사항 : -