-
미생물클러스터
-
MICROBIOTA
-
회원가입
-
ENG
-
자원 통합 검색
- 로그인
Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 18 : Chopped Meat Medium
| 조성 | 용량 | 단위 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Ground beef (fat free) | 500.0 | g | |||||||||
| Distilled water | 1000.0 | ml | |||||||||
| NaOH 1 N | 25.0 | ml | |||||||||
| Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: | |||||||||||
| Boil, cool under nitrogen atmosphere, add 0.5 g/l cysteine and adjust pH to 7.0. To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121?C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. | |||||||||||
| Casitone | 30.0 | g | |||||||||
| Yeast extract | 5.0 | g | |||||||||
| K2HPO4 | 5.0 | g | |||||||||
| Resazurin | 1.0 | mg | |||||||||
| Haemin solution (see below) | 10.0 | ml | |||||||||
|
|||||||||||
| Vitamin K1 or Vitamin K3 solution (see below) | 0.20 | ml | |||||||||
|
|||||||||||
** ph : 7.0.
** memo : -
** 추가조치 사항 : -
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스