KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
조성 | 용량 | 단위 | ||||||||||||||||||||||||||||||
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Artificial saltwater | 1.0 | L | ||||||||||||||||||||||||||||||
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Wolfe's mineral solution | 10.0 | ml | ||||||||||||||||||||||||||||||
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Mix components, distribute the medium into culture vessels (e.g., 10 ml in Balch-type tubes), seal with butyl rubber stoppers and autoclave. After autoclaving, aseptically add per liter the following solutions (filter-sterilized): | ||||||||||||||||||||||||||||||||
Trace vitamins (see Medium No. 1335) | 10.0 | ml | ||||||||||||||||||||||||||||||
8% NaHCO3 solution | 12.5 | ml | ||||||||||||||||||||||||||||||
0.2 M FeCl2 solution (freshly prepared) | 10.0 | ml | ||||||||||||||||||||||||||||||
Replace the gas phase with a N2-CO2-O2 (80:20:3, v/v/v) gas mixture and pressurize to 200 kPa with the same gas mixture. |
** ph : -
** memo : -
** 추가조치 사항 : **Trace minerals:
Nitrilotriacetic acid 1.5 g
MgSO4·7H2O 3.0 g
MnSO4·xH2O 0.5 g
NaCl 1.0 g
FeSO4·7H2O 0.1 g
CoSO4·7H2O 0.1 g
CaCl2·2H2O 0.1 g
ZnSO4·7H2O 0.1 g
CuSO4·5H2O 0.01 g
AlK(SO4)2 0.01 g
H3BO3 0.01 g
Na2MoO4·2H2O 0.01 g
Distilled water 1.0 L
Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.