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Distribution
조성 | 용량 | 단위 | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Beef extract | 10.0 | g | ||||||||||||||||||||||||
Peptone | 10.0 | g | ||||||||||||||||||||||||
Agar | 20.0 | g | ||||||||||||||||||||||||
Tap water | 250.0 | ml | ||||||||||||||||||||||||
Sea water· | 750.0 | ml | ||||||||||||||||||||||||
Artificial sea water: | ||||||||||||||||||||||||||
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** ph : -
** memo : Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature.
*Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.
** 추가조치 사항 : -