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** ph : 7.2
** memo : Inoculate `Rhizobium leguminosarum` KCTC1536 to TY liquid media (5g peptone, 3g yeast extract, 0.83g CaCl2?2H2O, L-1) and incubate at 30?C for 5 days by shaking with 100rpm.
Centrifuge the culture media at 8000rpm for 20min at 4C and collect the supernatant not to uptake the bacterial pellet, `Rhizobium leguminosarum`.
Mix thus obtained culture media and PY media (10g peptone, 6g yeast extract, 4g NaCl, L-1) with 1 : 1 volume and adjust pH to be 7.2.
Add gellan gum to the mixed media to be 1.5% and autoclave.
Pour the media approximately 15 to 20 mL to each petri dish.
Inoculate KCTC 32107 on the gellan gum media plate and incubate at 30C for 5 to 7 days.
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