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조성 | 용량 | 단위 |
---|---|---|
L-Glutamic acid | 2.0 | g |
Trisodium citrate·2H2O | 2.0 | g |
KH2PO4 | 1.0 | g |
NH4Cl | 1.0 | g |
K2SO4 | 5.0 | g |
MgCl2·6H2O | 20.0 | g |
NaCl | 200 | g |
Casamino acids (BD-Difco) | 4 | g |
Yeast extract (BD-Difco) | 2.0 | g |
0.2% FeSO4·6H2O solution | 2.0 | ml |
Trace metal solution(배지번호1686) | 2.0 | ml |
** ph : 5.2
** memo : For preparation of solid medium, add the components listed above (reduce the amount of NaCl to 150.0 g) to distilled water and bring volume to 800.0 ml. Separately prepare the following solution:
NaCl 50.0 g
Agar 20.0 g
Distilled water 200.0 ml
Combine both solutions after autoclaving.
** 추가조치 사항 : Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.0 with KOH.