KCTC Media No. 1225 : DESULFOBACTER SP. MEDIUM (LACTATE)

조성	용량	단위
Solution A:
조성 용량 단위 Na2SO4 3.00 g KH2PO4 0.2 g NH4Cl 0.3 g NaCl 21 g MgCl2·6 H2O 3.1 g KCl 0.5 g CaCl2 · 2 H2O 0.15 g Selenite-tungstate solution (see medium 1418) 1.0 ml Na-Resazurin solution(0.1% w/v) 0.5 ml Distilled water 920 ml
Solution B:	1.0	ml
조성 용량 단위 Trace element solution SL-10(see medium 1417) 1.0 ml
Solution C:
조성 용량 단위 Na2CO3 1.5 g Distilled water 30.0 ml
Solution D:
조성 용량 단위 Na-L-lactate 2.5 g Distilled water 10.0 ml
Solution E:
조성 용량 단위 Vitamin solution (see medium 1335) 10.0 ml
Solution F:
조성 용량 단위 Na2S · 9H2O 0.4 g Distilled water 10.0 ml

** ph : -

** memo : Solution A:
Na2SO4 3.00 g
KH2PO4 0.20 g
NH4Cl 0.30 g
NaCl 21.00 g
MgCl2 x 6 H2O 3.10 g
KCl 0.50 g
CaCl2 x 2 H2O 0.15 g
Selenite-tungstate solution (see medium 385) 1.00 ml
Resazurin 1.00 mg
Distilled water 870.00 ml
Solution B:
Trace element solution SL-10 (see medium 320) 1.00 ml
Solution C:
NaHCO3 5.00 g
Distilled water 100.00 ml
Solution D:
Na-L-lactate 2.50 g
Distilled water 10.00 ml
Solution E:
Vitamin solution (see medium 141) 10.00 ml
Solution F:
Na2S x 9 H2O 0.40 g
Distilled water 10.00 ml
Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under N2 gas atmosphere and sterilized by filtration. Solution B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4.
Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.

** 추가조치 사항 : Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at
least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type
tubes or serum vials and autoclaved. Solutions B, D and F are autoclaved separately under
100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere.
Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. To
complete the medium appropriate amounts of solutions B to F are added to the sterile
solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.