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조성 | 용량 | 단위 |
---|---|---|
NaCl | 120 | g |
MgCl2·6H2O | 6 | g |
KCl | 1.5 | g |
NaSO4 | 1.0 | g |
NH4Cl | 1.0 | g |
CaCl2·2H2O | 0.4 | g |
K2HPO4 | 0.4 | g |
Trace element solution(see medium 918) | 10.0 | ml |
Na-resazurin solution(0.1% w/v) | 0.5 | ml |
Na2CO3 | 2.5 | g |
D-Glucose | 1.0 | g |
Vitamin solution(see medium 1335) | 10.0 | ml |
L-Cysteine-HCl·H2O | 0.5 | g |
Na2S·9H2O | 0.5 | g |
D.W | 1.0 | L |
** ph : 7.5
** memo : Dissolve ingredients except carbonate, glucose, vitamins, cysteine and sulfide, then
sparge medium with 80% N2 and 20% CO2 gas mixture for 30 ? 45 min to make it
anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type
tubes or serum vials and autoclave. Add glucose, vitamins, cysteine and sulfide from
sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by
filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and
20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.5, if
necessary.
Note: Use at least 5-10%(v/v) inoculum.
** 추가조치 사항 : -