KCTC Media No. 1427 : Modified YCFA Medium
| 조성 |
용량 |
단위 |
| yeast extract |
4.0 |
g/L |
| casitone |
2.0 |
g/L |
| soy peptone |
2.0 |
g/L |
| NaHCO3 |
4 |
g/L |
| KH2PO4 |
0.41 |
g/L |
| MgCl2·6H2O |
0.1 |
g/L |
| CaCl2·2H2O |
0.11 |
g/L |
| Cysteine-HCl |
0.5 |
g/L |
| Vitamin K1 solution |
0.2 |
ml |
| 조성 |
용량 |
단위 |
| Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. |
|
|
|
| Haemin solution |
1.0 |
ml |
| 조성 |
용량 |
단위 |
| Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated. |
|
|
|
| trace elements I (alkaline) |
|
|
| 조성 |
용량 |
단위 |
| Na2SeO3 |
0.1 |
mM |
| Na2WO4 |
0.1 |
mM |
| Na2MoO4 |
0.1 |
mM |
| NaOH |
10.0 |
mM |
|
| trace elements II (acid) |
|
|
| 조성 |
용량 |
단위 |
| FeCl2 |
7.5 |
mM |
| H3BO4 |
1.0 |
mM |
| ZnCl2 |
0.5 |
mM |
| CuCl2 |
0.1 |
mM |
| MnCl2 |
0.5 |
mM |
| CoCl2 |
0.5 |
mM |
| NiCl2 |
0.1 |
mM |
| HCl |
50 |
mM |
|
| vitamin solution |
|
|
| 조성 |
용량 |
단위 |
| biotin |
0.02 |
g/L |
| niacin |
0.2 |
g/L |
| pyridoxine |
0.5 |
g/L |
| riboflavin |
0.1 |
g/L |
| thiamine |
0.2 |
g/L |
| cyanocobalamin |
0.1 |
g/L |
| p-aminobenzoic acid |
0.1 |
g/L |
| pantothenic acid |
0.1 |
g/L |
|
** ph : 7.0
** memo : The medium was supplemented with 30mM of sodium acetate. For routine use, the medium was distributed in 35 ml serum bottles sealed with butyl-rubber stoppers at 37 °C under a gas phase of 1.7 atm (172 kPa) N2/CO2 (80 : 20, v/v). The pH of the medium was 7.0.
** 추가조치 사항 : -
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