KCTC Media No. 1454 : MMJS MEDIUM

조성	용량	단위
NaCl	20.0	g
K2HPO4	0.14	g
CaCl2	0.8	g
NH4Cl	1.0	g
MgSO4·7H2O	4.0	g
MgCl2·6H2O	3.0	g
KCl	0.33	g
NiCl2·6H2O	0.5	mg
Na2SeO3·5H2O	0.5	mg
Na2WO4	0.1	mg
Fe(NH4)2(SO4)2·6H2O	0.01	g
trace mineral solution(see the medium No.918)	10.0	ml
vitamin solution(see the medium No.1335)	10.0	ml
NaHCO3	0.3	g

** ph : 5.5

** memo : NaHCO3 were dissolved, and the pH of the medium was adjusted to
pH 5.5 with HCl before autoclaving. After autoclaving
under an air atmosphere, a filter-sterilized and concentrated
solution of vitamins and NaHCO3 was added to the
medium under gas purging with 80% N2/20% CO2, and
the pH was readjusted to pH 6.0 with HCl at room temperature,
if necessary.
MMJS medium consists of MMJH medium with 5 mM Na2
S2O3
5H2O, and the gas phase in the vials was adjusted to
94% N2/5% CO2/1% O2 (200 kPa).

** 추가조치 사항 : NaHCO3 were dissolved, and the pH of the medium was adjusted to
pH 5.5 with HCl before autoclaving. After autoclaving
under an air atmosphere, a filter-sterilized and concentrated
solution of vitamins and NaHCO3 was added to the
medium under gas purging with 80% N2/20% CO2, and
the pH was readjusted to pH 6.0 with HCl at room temperature,
if necessary.
MMJS medium consists of MMJH medium with 5 mM Na2
S2O3
5H2O, and the gas phase in the vials was adjusted to
94% N2/5% CO2/1% O2 (200 kPa).