KCTC Media No. 1500 : MARINE THERMOCOCCUS MEDIUM

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** memo : Medium No. 187 is than used to make Medium No. 1500 according to the protocol described at:

https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium760.pdf
using nitrogen as gas phase and pH 7.0.


Steps in detail:

Prepare liquid Bacto Marine Broth (see medium 187) and filter through normal filter
paper. An iron sediment will collect in the filter. Boil the medium in a water bath and cool
under nitrogen gas (keep anaerobic). Adjust pH to 7.0.
Dispense the medium into Hungate tubes or serum bottles containing finely divided
sulphur (0.5% w/v), seal the tubes or bottles under the same anaerobic gas used when
cooling the medium. Sterilise the medium at 100℃ for 3 hours on 3 consecutive days.
Reduce the medium by adding 10% neutralised sodium sulphide to a final concentration
of 0.05 %. The medium should not give a heavy black precipitate, if it does the iron
sediment was not adequately removed by filtering in the initial stages and the medium
should be made again, making sure that the iron is removed by filtering.

** 추가조치 사항 : -