KCTC Media No. 1506 : PSEUDOCLOSTRIDIUM MEDIUM

조성	용량	단위
NaCl	1.2	g
MgCl2·6H2O	0.4	g
KCl	0.3	g
CaCl2·2H2O	0.15	g
NH4Cl	0.27	g
KH2PO4	0.21	g
Na2SO4	0.1	g
Trace element solution SL-10 (see medium 1417)	1.0	ml
Inulin	5.0	g
Na-resazurin solution (0.1% w/v)	0.5	ml
Yeast extract	0.03	g
Casamino acids (BD Bacto)	0.03	g
Vitamin solution (see medium 1335)	10.0	ml
Na2CO3	1.5	g
Na2HPO4	2.66	g
Na2S·9H2O	0.15	g
Distilled water	1000	ml

** ph : 7.0

** memo : Dissolve ingredients (except yeast extract, Casamino acids, vitamins, carbonate,
hydrogenphosphate and sulfide), bring medium to the boil, then cool to room
temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas
atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After
sterilization add yeast extract, Casamino acids, hydrogenphosphate and sulfide from
sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile
anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are
prepared under 100% N2 gas atmosphere and strerilized by filtration. Adjust pH of
complete medium to 7.0, if necessay.

** 추가조치 사항 : -