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Distribution
조성 | 용량 | 단위 |
---|---|---|
Lab-Lemco powder (Oxoid) | 2.4 | g |
Proteose peptone No.3 (BD-Difco) | 10.0 | g |
Yeast extract (BD-Difco) | 5.0 | g |
Na2HPO4 | 4 | g |
Glucose | 1.5 | g |
Soluble starch | 0.5 | g |
L-Cystine | 0.2 | g |
Agar | 15.0 | g |
L-Cysteine·HCl·H_2O | 0.5 | g |
Horse blood | 50 | ml |
Distilled water | 950 | ml |
** ph : 7.5
** memo : -
** 추가조치 사항 : Add L-cystine to 50ml of 1N HCl and mix thotoughly. Add the remaining components and bring volume to 950ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0ml of horse blood. Mix thotoughly and pour into sterile petri dished or distribute into sterile tubes.