KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
조성 | 용량 | 단위 | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
KH2PO4 | 0.136 | g | |||||||||||||||
NH_4Cl | 0.535 | g | |||||||||||||||
MgCl_2·6H2O | 0.204 | g | |||||||||||||||
CaCl_2·2H2O | 0.147 | g | |||||||||||||||
Sodium acetate | 0.82 | g | |||||||||||||||
Na2SO4 | 0.142 | g | |||||||||||||||
Trace element solution(see Medium No.1475) | 10.0 | ml | |||||||||||||||
Trace vitamins (see Medium No.1335) | 10.0 | ml | |||||||||||||||
Se/W solution | 1.0 | ml | |||||||||||||||
|
|||||||||||||||||
Distilled water | 910 | ml | |||||||||||||||
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, add aseptically and anaerobically the following solutions to the medium: | |||||||||||||||||
8% NaHCO_3 solution (filter-sterilized) | 30.0 | ml | |||||||||||||||
··100 mM Fe NTA solution | 50 | ml | |||||||||||||||
|
|||||||||||||||||
Adjust pH to 7.0 - 7.2, if necessary. | |||||||||||||||||
1.0 M Sodium fumarate solution | 10.0 | ml |
** ph : -
** memo : -
** 추가조치 사항 : **100 mM Fe NTA solution can be replaced with Iron(III) citrate solution (see below)
Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved.
Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution.
Fill up to 100 ml with distilled water.