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Distribution
조성 | 용량 | 단위 |
---|---|---|
KH2PO_4 | 0.33 | g/L |
NH4Cl | 0.33 | g/L |
KCl | 0.33 | g/L |
MgCl2·6H2O | 0.33 | g/L |
CaCl2·6H2O | 0.033 | g/L |
NaCl | 10.0 | g/L |
NaHCO3 | 2.0 | g/L |
trace-elements ( KCTC Medium No. 1571) | 1.0 | ml/L |
vitamins ( KCTC Medium No. 1569) | 1.0 | ml/L |
resasurin solution | 0.0002 | % |
NaHCO3 and vitamins, Na2Sx·9H2O (0.5g/L) are added after boiling and colling of the medium under 80% N2: 20% CO2. Adgust the pH 7.5 with sterile HCl or NaOH after autoclaving. |
** ph : 7.5
** memo : -
** 추가조치 사항 : - 10mM sodium formate and 10mM sodium thiosulfate with addition of at least 50mg/l yeast extract from separately prepared sterile stocks are used as optimal growth substrates. The incubation time is 4-6 days.
- 3,4-ihydroxybenzoic acid (10mM, adjust pH to neutral with NaOH!) with addition of at least 50mg/l yeast extract from separately prepared sterile stocks is also can be used as a growth substriate.