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Distribution
조성 | 용량 | 단위 |
---|---|---|
Yeast extract | 0.05 | g/L |
Fish Peptone | 0.25 | g/L |
Sodium formate | 0.2 | g/L |
Sodium acetate | 0.2 | g/L |
Sodium lactate | 0.2 | g/L |
Sodium pyruvate | 0.2 | g/L |
Sodium succinate | 0.2 | g/L |
Malic acid | 0.2 | g/L |
Fumaric acid | 0.2 | g/L |
Sodium citrate | 0.2 | g/L |
KCl | 5.4 | g/L |
K2HPO4 | 0.2 | g/L |
CaCl_2 | 0.29 | g/L |
NH_4Cl | 0.27 | g/L |
MgSO_4·7H_2O | 26.8 | g/L |
MgCl_2·6H_2O | 23 | g/L |
NaCl | 184 | g/L |
Washed Agar | 15-18 | g/L |
Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 1 M NaOH. |
** ph : 7.0
** memo : Uploading the HCM 7.0 medium information received from the depositor to the document for KCTC 4305.
KCTC4331, 4332 배양시 Fish peptone 대신 Peptone, Sodium citrate 대신 Citric acid 사용하였음.
** 추가조치 사항 : Washed agar
Add 20.0 g pure agar power to 400 ml distilled water. Mix well for 5 min then allow the agar to settle for 30 min, decant the supernatant. Repeat washing as in the previous step until the supernatant is clear.