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Distribution
조성 | 용량 | 단위 | |||||||||||||||
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MgSO_4·7H_2O | 1.0 | g | |||||||||||||||
CaCl_2·2H_2O | 0.2 | g | |||||||||||||||
KNO_3 | 0.25 | g | |||||||||||||||
FeCl_2 solution | 1.0 | ml | |||||||||||||||
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Trace element solution (SL-10, KCTC Medium No.1417) | 1.0 | ml | |||||||||||||||
1 M HEPES solution (pH 7.0) | 2.0 | ml | |||||||||||||||
Ammonium ferric citrate | 4 | mg | |||||||||||||||
Distilled water | 1.0 | L | |||||||||||||||
Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved) to the medium: | |||||||||||||||||
Phosphate buffer solution | 2.0 | ml | |||||||||||||||
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Vitamin solution | 10.0 | ml | |||||||||||||||
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** ph : 6.8
** memo : -
** 추가조치 사항 : Adjust pH to 6.8, if necessary. Cultivate with methane or methanol as a substrate as described below:
Methane: Aseptically distribute the medium into sterile serum bottles (leave ≥ 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.