KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
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** ph : 7.0--7.2
** memo : -
** 추가조치 사항 : Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with
80% N2 and 20% CO2 gas mixture for 30 ? 45 min to make it anoxic. Add and dissolve
bicarbonate, adjust pH to 7.0, then dispense medium under 80% N2 and 20% CO2 gas
atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins
and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin
solution should be sterilized by filtration. Prior to use check pH of complete medium and
adjust to 7.0 - 7.2, if necessary.