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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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자원 통합 검색
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 1842 : Medium for Thiorhodovibrio frisius 970 (SL12, saline)
| 조성 | 용량 | 단위 |
|---|---|---|
| KH2PO4 | 0.25 | g/L |
| NH_4Cl | 0.34 | g/L |
| KCl | 0.34 | g/L |
| MgSO_4·7H_2O | 0.5 | g/L |
| CaCl_2·2H_2O | 0.25 | g/L |
| NaCl | 20.0 | g/L |
| MgCl_2·6H_2O | 2.8 | g/L |
| dissolve components in double distilled water --> autoclave in Widdel flask | ||
| NaHCO_3 | 1.5 | g/L |
| dissolve it in ~50ml double distilled water in a 100ml Pfennig bottle, gas it for 15 min with CO_2, autoclave bottle capped tightly | ||
| ·Procedure after autoclaving | ||
| gas flask directly after taking out of the autoclave with N2 while cooling down in a beaker with ice on a magnetic stirrer | ||
| ~10 min with cap little opened , stirrer off | ||
| till cooling down to RT with caps closed, stirrer on | ||
| ·Addition of solutions after cooling down: | ||
| NaHCO_3 (prepared in Pfennig bottle) | ||
| SL12B mit EDTA | 1.0 | ml/L |
| Vitamine B12 (2mg/100ml) | 1.0 | ml/L |
| 10 Vit.-sol (1000x) | 1.0 | ml/L |
| Na2S ( 1M Stocksol.) | 1.25 | ml/L |
** ph : 7.2
** memo : -
** 추가조치 사항 : -
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스