Peptone |
0.25 |
g |
Yeast extract |
0.25 |
g |
(4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid) (HEPES) (10 mM) |
2.38 |
g |
artificial seawater (ASW) |
250 |
ml |
조성 |
용량 |
단위 |
NaCl |
46.94 |
g/L |
Na2SO4 |
7.84 |
g/L |
MgCl2_·6H_2O |
21.28 |
g/L |
CaCl_2·2H2_O |
2.86 |
g/L |
NaHCO_3 |
0.384 |
g/L |
KCl |
1.384 |
g/L |
KBr |
0.192 |
g/L |
H3BO3 |
0.052 |
g/L |
SrCl_2·6H_2O |
0.08 |
g/L |
NaF |
0.006 |
g/L |
|
Double distilled water (final volume) |
973 |
ml |
The pH was adjusted to 7.0 using 5 M KOH and the solution was autoclaved for 20 min at 121 °C. After cooling, the following solutions were added aseptically: |
|
|
조성 |
용량 |
단위 |
25% (w/v) glucose |
1.0 |
ml |
vitamin solution |
5.0 |
ml |
trace element solution |
1.0 |
ml |
stock solution with 50 g/L N-acetyl glucosamine (NAG) |
20.0 |
ml |
|
· Vitamin solution |
|
|
조성 |
용량 |
단위 |
p-aminobenzoic acid |
10.0 |
mg/L |
biotin |
4 |
mg/L |
pyridoxine hydrochloride |
20.0 |
mg/L |
thiamine hydrochloride |
10.0 |
mg/L |
Ca-pantothenate |
10.0 |
mg/L |
folic acid |
4 |
mg/L |
riboflavin |
10.0 |
mg/L |
nicotinamide |
10.0 |
mg/L |
vitamin B12 |
0.2 |
mg/L |
p-Aminobenzoic acid was dissolved first and the solution was sterilised by filtration and stored in the dark at 4 °C. |
|
|
|
· Trace element solution |
|
|
조성 |
용량 |
단위 |
Na-nitrilotriacetate |
1.5 |
g/L |
MnSO_4·H_2O |
500 |
mg/L |
FeSO4·7H2_O |
100 |
mg/L |
Co(NO_3)_2·6H_2O |
100 |
mg/L |
ZnCl_2 |
100 |
mg/L |
NiCl_2·6H_2O |
50 |
mg/L |
H2SeO3 |
50 |
mg/L |
CuSO_4?×?5H_2O |
10.0 |
mg/L |
AlK(SO_4)_2·12H_2O |
10.0 |
mg/L |
H3BO3 |
10.0 |
mg/L |
NaMoO_4·2H_2O |
10.0 |
mg/L |
Na2WO4·2H_2O |
10.0 |
mg/L |
was sterilised by filtration and stored in the dark at 4 °C. |
|
|
|