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Distribution
조성 | 용량 | 단위 |
---|---|---|
NaCl | 20.0 | g/L |
MgCl2 x 6 H2O | 3.0 | g/L |
KH2PO_4 _ | 1.0 | g/L |
Na3-citrate x 5 H2O | 3.0 | g/L |
Yeast extract (OXOID) | 5.0 | g/L |
Peptone (BD Bacto) | 2.0 | g/L |
Trace element solution SL-10 (see medium 1417) | 1.0 | ml/L |
Sodium resazurin (0.1% w/v) | 0.05 | ml/L |
CaCl2 x 2 H2O | 0.2 | g/L |
Na2S2O3 x 5 H2O | 2.5 | g/L |
Na2S x 9 H2O | 0.5 | g/L |
Distilled water | 1.0 | L |
Dissolve ingredients except calcium chloride, thiosulfate and sulfide. Sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride, thiosulfate and sulfide from sterile anoxic stock solutions prepared under 100% N 2 gas. The pH of the complete medium should be at 6.7 - 6.8. |
** ph : -
** memo : -
** 추가조치 사항 : -