Solution B: |
|
|
조성 |
용량 |
단위 |
Thiamine pyrophosphate |
6 |
mg |
NaHCO3 |
2.0 |
g |
Distilled water |
25 |
ml |
volatile fatty acid solution |
20.0 |
ml |
Sterilize by filtration, add 20 ml rabbit serum, gas with 80% N2 + 20% CO2. |
|
|
|
volatile fatty acid solution |
|
|
조성 |
용량 |
단위 |
0.1 N KOH |
100 |
ml |
Isobutyric acid |
0.5 |
ml |
D,L-2-Methylbutyric acid |
0.5 |
ml |
Isovaleric acid |
0.5 |
ml |
Valeric acid |
0.5 |
ml |
|
Solution A: |
|
|
조성 |
용량 |
단위 |
Heart infusion broth |
12.5 |
g |
Trypticase peptone (BBL11921) |
10.0 |
g |
Yeast extract |
2.5 |
g |
Sodium thioglycolate |
0.5 |
g |
L-Cysteine·HCl·H2O |
1.0 |
g |
L-Asparagine |
0.25 |
g |
Glucose |
2.0 |
g |
Agar |
3.0 |
g |
Distilled water |
1000 |
ml |
Boil for 5 min, cool to 50?C while gassing with 80% N2 + 20% CO2. Distribute to
anaerobic tubes under the same atmosphere and autoclave. |
|
|
|
Add 0.45 ml Sol. B to 10 ml Sol. A. Inoculate and pressurize the tubes with H2 to 0.5
bar. |
|
|