KCTC Media No. 230 : Desulfobacter postgatei Medium

조성	용량	단위
Solution A:
조성 용량 단위 Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g KCl 0.50 g Resazurin 1.00 mg Lipoic acid 5.000 mg MgCl2 . 6H2O 1.30 g CaCl2 . 2H2O 0.15 g Distilled water 870.00 ml
Solution B:
조성 용량 단위 Trace element solution SL-10 (see below) 1.00 ml
Solution C:
조성 용량 단위 NaHCO3 5.00 g Distilled water 100.00 ml
Solution D:
조성 용량 단위 Na-acetate . 3H2O 2.50 g Distilled water 10.00 ml
Solution E:
조성 용량 단위 Vitamin solution (see below) 10.0 ml
Solution F:
조성 용량 단위 Na2S . 9H2O 0.40 g Distilled water 10.00 ml
Trace element solution SL-10 :
조성 용량 단위 HCl (25%; 7.7 M) 10.00 ml ZnCl2 70.00 mg H3BO3 6.00 mg MnCl2 . 4H2O 100.00 mg NiCl2 . 6H2O 24.00 mg Na2MoO4 . 2H2O 36.00 mg CoCl2 . 6H2O 190.00 mg CuCl2 . 2H2O 2.00 mg FeCl2 . 4H2O 1.50 g Distilled water 990.0 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Vitamin solution :
조성 용량 단위 Biotin 2.000 mg Folic acid 2.000 mg Pyridoxine-HCl 10.000 mg Riboflavin 5.000 mg Nicotinic acid 5.000 mg D-Ca-pantothenate 5.000 mg Vitamin B12 0.100 mg p-Aminobenzoic acid 5.000 mg Thiamine-HCl . 2H2O 5.000 mg Distilled water 1000.000 ml

** ph : 7.1--7.4

** memo : Solution A is boiled for a few minutes, cooled to room temperature, gassed with 80% N_2_+ 20% CO_2_ gas mixture to reach a pH below 6, then autoclaved anaerobically under the same gas mixture. Solutions B, D, E and F are autoclaved separately under nitrogen. Solution C is filter-sterilized and flushed with 80% N_2_ + 20% CO_2_ to remove dissolved oxygen. Solution B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anaerobically under 80% N_2_ + 20% CO_2_ into appropriate vessels. Final pH of the medium is 7.1 - 7.4.
Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N_2_ and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum.

** 추가조치 사항 : -