KCTC Media No. 270 : Minimal Salts (MS) Medium
조성 |
용량 |
단위 |
Solution A |
50 |
ml/L |
조성 |
용량 |
단위 |
KH2PO4 |
8 |
g/L |
MgCl·6H2O |
4 |
g/L |
NH4Cl |
5.0 |
g/L |
CaCl2·2H2O |
1.0 |
g/L |
|
Solution B |
50 |
ml/L |
|
Trace metal solution |
10.0 |
ml/L |
조성 |
용량 |
단위 |
Trace metal solution (100X) |
|
|
Nitrilotriacetic acid |
12.8 |
g/L |
FeSO4·7H2O |
0.42 |
g/L |
MnCl2·4H2O |
0.1 |
g/L |
CoCl2·6H2O |
0.17 |
g/L |
CuCl2·2H2O |
0.02 |
g/L |
ZnSO4·7H2O |
0.21 |
g/L |
Na2MoO4·2H2O |
0.01 |
g/L |
|
Wolfe's vitamin solution |
10.0 |
ml/L |
조성 |
용량 |
단위 |
Wolfe's vitamin solution (100X) |
|
|
Biotin |
2.0 |
mg/L |
folic acid |
2.0 |
mg/L |
pyridoxine HCl |
10.0 |
mg/L |
thiamine HCl |
5.0 |
mg/L |
riboflavin |
5.0 |
mg/L |
nicotinic acid |
5.0 |
mg/L |
calcium D-(+)pantothenate |
5.0 |
mg/L |
cyanocobalamine |
0.1 |
mg/L |
p-aminobenzoic acid |
5.0 |
mg/L |
thioctic acid |
5.0 |
mg/L |
|
Yeast extracrt |
1.0 |
g/L |
Resazurin(0.1%) |
1.0 |
ml/L |
NaHCO3 |
3.52 |
g/L |
** ph : 7-7.2
** memo : Adjust it with NaOH
Final concentration of ~0.5% colloidal chitin was mixed with MS medium
as sole carbon substrate to grow the strain.
** 추가조치 사항 : -