KCTC Media No. 282 : Modified PYG-Medium
| 조성 |
용량 |
단위 |
| Trypticase peptone |
5.0 |
g |
| Peptone |
5.0 |
g |
| Yeast extract |
10.0 |
g |
| Beef extract |
5.0 |
g |
| Glucose |
5.0 |
g |
| K2HPO4 |
2.0 |
g |
| Tween 80 |
1.0 |
ml |
| Resazurin |
1.0 |
mg |
| L-cycsteine,HCl |
0.5 |
g |
| Vitamin K1solution |
0.2 |
ml |
| 조성 |
용량 |
단위 |
| Dissolve 0.1 ml of vitamin K1 in 20 ml 95 % ethanol and filter sterilize. Store refrigerated in a brown bottle. |
|
|
|
| Salt solution |
40.0 |
ml |
| 조성 |
용량 |
단위 |
| K2HPO4 |
1.0 |
g |
| KH2PO4 |
1.0 |
g |
| NaHCO3 |
10.0 |
g |
| NaCl |
2.0 |
g |
| CaCl2 . 2H2O |
0.25 |
g |
| MgSO4 . 7H2O |
0.5 |
g |
| Distilled water |
1.0 |
L |
|
| Distilled water |
950.0 |
ml |
| Haemin solution |
10.0 |
ml |
| 조성 |
용량 |
단위 |
| Dissolve 50 mg hemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated. |
|
|
|
** ph : 7.2
** memo : Vitamin K_1_, hemin solution, and cysteine are added after the medium has been boiled and cooled under CO_2_. Adjust pH to 7.2 using 8 N NaOH. Distribute under N_2_ and autoclave.
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