KCTC Media No. 358 : Bacillus acidoterrestris and Bacillus Cycloheptanicus Medium
| 조성 |
용량 |
단위 |
| Solution A: |
|
|
| 조성 |
용량 |
단위 |
| (NH4)2SO4 |
0.2 |
g |
| Yeast extract |
2.0 |
g |
| Glucose |
5.0 |
g |
| KH2PO4 |
3.0 |
g |
| Distilled water(for liquid medium) |
1.0 |
L |
| (for solid medium) |
500.0 |
ml |
| Adjust pH to 4.0 |
|
|
| CaCl2 . H2O |
0.25 |
g |
| MgSO4 . 7H2O |
0.5 |
g |
|
| Solution B: |
|
|
| 조성 |
용량 |
단위 |
| Trace element solution SL-6 (see medium 339) |
1.0 |
ml |
|
| Solution C: |
|
|
| 조성 |
용량 |
단위 |
| Distilled water |
500.0 |
ml |
| Agar |
15,.0 |
g |
|
** ph : 4.5
** memo : Sterilize separately.
For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C.
For strains of `Bacillus cycloheptanicus` add 5 g/l of yeast extract instead of 2 g/l.
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