Solution A: |
|
|
조성 |
용량 |
단위 |
Yeast extract |
2.00 |
g |
Glucose |
5.00 |
g |
Distilled water (for liquid medium) |
1000.00 |
mg/L |
Distilled water (for solid medium) |
500.00 |
mg/L |
Adjust pH to 4.0 |
|
|
CaCl2 . 2H2O |
0.25 |
g |
MgSO4 . 7H2O |
0.50 |
g |
(NH4)2 SO4 |
0.20 |
g |
KH2PO4 |
3.00 |
g |
|
Solution B: |
|
|
조성 |
용량 |
단위 |
Trace element sol. SL-6 |
1.00 |
ml |
|
Solution C: |
|
|
조성 |
용량 |
단위 |
Agar |
15.00 |
g |
Distilled water |
500.00 |
ml |
|
Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and
solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and
solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l. |
|
|
Trace element solution SL-6: |
|
|
조성 |
용량 |
단위 |
Distilled water |
1000.00 |
ml |
ZnSO4 . 7H2O |
0.10 |
g |
CoCl2 . 6H2O |
0.20 |
g |
NiCl2 . 6H2O |
0.02 |
g |
MnCl2 . 4H2O |
0.03 |
g |
H3BO3 |
0.30 |
g |
CuCl2 . 2H2O |
0.01 |
g |
Na2MoO4 . 2H2O |
0.03 |
g |
|