KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
|Filtered, aged seawater (see below)||750.0||ml|
** ph : 7.3
** memo : Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3.
Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Cool to 50°C and add warm (50°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature.
*Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.