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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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자원 통합 검색
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 768 : CLOSTRIDIUM CHARTATABIDUM MEDIUM
| 조성 | 용량 | 단위 | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mineral solution (see below) | 38.00 | ml | |||||||||||||||||||||
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| K2HPO4 | 0.30 | g | |||||||||||||||||||||
| Trypticase peptone (BBL) | 2.00 | g | |||||||||||||||||||||
| Yeast extract(OXOID) | 0.50 | g | |||||||||||||||||||||
| Haemin solution(0.05% w/v, see medium 704) | 2.0 | ml | |||||||||||||||||||||
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| Cellobiose | 5.0 | g | |||||||||||||||||||||
| Na2CO3 | 4.0 | g | |||||||||||||||||||||
| Na-resazurin solution (0.1% w/v) | 0.5 | ml | |||||||||||||||||||||
| L-Cysteine-HCl·H2O | 0.25 | g | |||||||||||||||||||||
| Na2S·9H2O | 0.25 | g | |||||||||||||||||||||
| Distilled water | 760 | ml | |||||||||||||||||||||
| Clarified rumen fluid | 200 | ml | |||||||||||||||||||||
| Dissolve ingredients (except carbonate, cellobiose, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N 2 gas. Cellobiose has to be sterilized by filtration. Adjust pH of complete medium to 6.7 - 6.8, if necessary. | |||||||||||||||||||||||
** ph : -
** memo : -
** 추가조치 사항 : Clarified rumen fluid :
Rumen fluid from cow or sheep (obtained from fistulated animals or abattoir refuse) is
filtered through muslin, autoclaved at 121°C for 15 min and then centrifuged at 27,000 g
for 20 min. The supernatant is made anoxic by sparging with 100% N2 gas for 15 min,
dispensed under same gas atmosphere into anoxic serum vials to 30% of volume and then
stored frozen at -20°C.
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스