KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
조성 | 용량 | 단위 | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Trypticase peptone (BD BBL) | 5.0 | g | ||||||||||||||||||||||||
Peptone from meat (pepsin-digested) | 5.0 | g | ||||||||||||||||||||||||
Yeast extract | 10.0 | g | ||||||||||||||||||||||||
D-Glucose | 5.0 | g | ||||||||||||||||||||||||
Na-Resazurin solution(0.1% w/v) | 0.5 | ml | ||||||||||||||||||||||||
Salt solution (see below) | 40.0 | ml | ||||||||||||||||||||||||
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L-Cysteine-HCl·H2O | 0.5 | g | ||||||||||||||||||||||||
Distilled water | 1000.0 | ml |
** ph : -
** memo : For KCTC5587 replace glucose with 5.0 g/l xylan.
For KCTC5426, KCTC15432 omit glucose.
** 추가조치 사항 : Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2
gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense
medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials
and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic
stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete
medium to 6.8 - 7.0, if necessary.