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Distribution
조성 | 용량 | 단위 |
---|---|---|
NH4Cl | 1.0 | g |
MgCl2·6H2O | 1.0 | g |
CaCl2·2H2O | 0.4 | g |
K2HPO4·3H2O | 0.4 | g |
Trace element solution (see medium 539) | ||
Trypticase | ||
Yeast extract | ||
NaHCO3 | ||
Gelatine | ||
Resazurin | ||
Na2S·9H2O | ||
Distilled water |
** ph : -
** memo : Prepare medium anaerobically under 100% CO_2_. Prior to autoclaving in closed tubes or
bottles, flush medium with CO_2_ for 20 min and adjust pH to 7.0.
Sterilize separate solutions of gelatine (3g/100 ml) and sodium sulfide (0.5g/10 ml) by
autoclaving under N_2_. Add the required volumes to complete the medium before use.
For KCTC 5673 : Use 5g/l of glucose as the substrate replacing gelatine.
** 추가조치 사항 : -