KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
조성 | 용량 | 단위 | ||||||||||||||||||||||||||||||||||||||||||
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KH2PO4 | 1.5 | g/L | ||||||||||||||||||||||||||||||||||||||||||
Na2HPO4·12H2O | 4.2 | g/L | ||||||||||||||||||||||||||||||||||||||||||
NH4Cl | 0.5 | g/L | ||||||||||||||||||||||||||||||||||||||||||
MgCl2·6H2O | 0.18 | g/L | ||||||||||||||||||||||||||||||||||||||||||
Yeast extract (Difco 0127) | 2.0 | g/L | ||||||||||||||||||||||||||||||||||||||||||
Glucose^·^ | 8.0 | g/L | ||||||||||||||||||||||||||||||||||||||||||
Vitamin solution (see below) | 0.5 | ml/L | ||||||||||||||||||||||||||||||||||||||||||
Wolfe''s modified mineral elixir | 5.0 | ml/L | ||||||||||||||||||||||||||||||||||||||||||
Resazurin (0.1 %) | 1.0 | ml/L | ||||||||||||||||||||||||||||||||||||||||||
Reducing solution : | 40.0 | ml/L | ||||||||||||||||||||||||||||||||||||||||||
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The sodium hydroxide solution is brought to boiling and made anaerobic by bubbling with N2/H2 (95/5 %) gas mixture. The solution is allowed to cool and the sodium sulfide and cysteine are then added. Under anaerobic conditions, the solution is tubed, stoppered and autoclaved for 15 minutes at 15 psi. | ||||||||||||||||||||||||||||||||||||||||||||
Vitamin solution: | ||||||||||||||||||||||||||||||||||||||||||||
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The vitamins are dissolved in 500 ml of water. Of this solution, 0.05 ml is added per 100 ml of medium. The vitamin solution is best stored frozen or in a refrigerator in the absence of light. | ||||||||||||||||||||||||||||||||||||||||||||
Wolfe's modified mineral elixir: | ||||||||||||||||||||||||||||||||||||||||||||
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The nitriloacetic acid is suspended in 500 ml water. It is dissolved by titrating with 2--3N KOH until the pH is stabilized at 6.5. The rest of the ingredients are then added and dissolved in the order they are listed. Finally the volume is adjusted to 1000 ml. For 100 ml of medium, use 5.0 ml of the mineral elixir. When preparing tubed medium, Whatman #1 filter paper may be utilized. In this case, one strip (8 x 70 mm) per tube of broth" is used. |
** ph : -
** memo : A note of caution : this medium contains sodium sulfide, and hydrogen sulfide production will occur, especially
upon prolonged boiling. Hydrogen sulfide is hazardous and preparation of this medium should be done in a chemical
fume hood. The medium is brought to boiling while being gassed with
a mixture of 95 % N_2_ and 5 % H_2_. The solution is kept boiling for several minutes in which time the color turns from blue
to reddish-pink. During continuous gassing and boiling, the reducing solution is added. The pink color should disappear, indicating reduction. The medium need only be boiled briefly
for reduction. Using anaerobic (Hungate) technique, the medium is dispensed in tubes previously made anaerobic by
flushing with N_2_/H_2_ gas mixtures at 15 psi.
** 추가조치 사항 : -