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|CaCl2· 2 H2O||0.13||g|
|Na2-ß-glycerol phosphate· 5 H2O||6.00||g|
|FeSO4· 7 H2O solution (0.1% w/v in 0.1 N H2SO4)||1.10||ml|
|Na-resazurin solution (0.1% w/v)||0.5||ml|
|Dissolve ingredients except cellobiose, sparge medium with 80% N2 and 20% CO2 gas mixture for 30 ? 45 min to make it anoxic. Then adjust pH to 7.0 - 7.2, distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Cellobiose is added to the sterile medium from an anoxic 10% (w/v) stock solution prepared under 100% N2 gas and sterilized by filtration. Some strains can be adapted to cellulose as substrate using 10.00 g/l cellulose (Avicel or MN 301, MACHEREY-NAGEL). Note: A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth.|
** ph : -
** memo : DSM 122 medium