KCTC Media No. 626 : HETEROTROPHIC MEDIUM H3P
| 조성 |
용량 |
단위 |
| Solution A: |
|
|
| 조성 |
용량 |
단위 |
| Distilled water |
50.000 |
ml |
| KH2PO4 |
2.300 |
g |
| Na2HPO4 . 2H2O |
2.900 |
g |
|
| Solution B: |
|
|
| 조성 |
용량 |
단위 |
| Trace element sol. SL-6 (see medium 232) |
5.000 |
ml |
| Distilled water |
850.000 |
ml |
| Agar (if necessary) |
15.000 |
g |
| NH4Cl |
1.000 |
g |
| CaCl2 . 2H2O |
0.010 |
g |
| MgSO4. 7H2O |
0.500 |
g |
| MnCl2 . 4H2O |
0.005 |
g |
| NaVO3 . H2O |
0.005 |
g |
|
| Solution C: |
|
|
| 조성 |
용량 |
단위 |
| Ferric ammonium citrate |
0.050 |
g |
| Distilled water |
20.000 |
ml |
|
| Solution D: |
|
|
| 조성 |
용량 |
단위 |
| Yeast extract |
1.000 |
g |
| Na-acetate |
1.000 |
g |
| DL-Malate |
1.000 |
g |
| Distilled water |
30.000 |
ml |
| pH 7.0 |
|
|
| Na2-succinate |
1.000 |
g |
|
| Solution E: |
|
|
| 조성 |
용량 |
단위 |
| Na-lactate |
1.000 |
g |
| Na-pyruvate |
1.000 |
g |
| D-Mannitol |
1.000 |
g |
| D-Glucose |
2.000 |
g |
| Distilled water |
50.000 |
ml/L |
| pH 7.0 |
|
|
|
| Standard vitamin solution: |
|
|
| 조성 |
용량 |
단위 |
| Riboflavin |
10.000 |
mg |
| Nicotinic acid |
50.000 |
mg |
| Pyridoxine-HCl |
50.000 |
mg |
| Ca-pantothenate |
50.000 |
mg |
| Biotin |
0.100 |
mg |
| Folic acid |
0.200 |
mg |
| Distilled water |
100.000 |
ml |
| Thiamine-HCl . 2H2O |
50.000 |
mg |
| Vitamin B12 |
1.000 |
mg |
|
** ph : 7.0
** memo : H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of
aerobic bacteria (Ref. 3367).
Solutions A, B, C and D are autoclaved separately for 15 min at 121°C, cooled down to 50°C and then
mixed aseptically with filter-sterilized solution E (at 50°C) and 5.0 ml of standard vitamin
solution. The final pH of this medium should be 6.8 without adjustment
** 추가조치 사항 : -
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