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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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자원 통합 검색
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 990 : ANAEROCELLUM MEDIUM
| 조성 | 용량 | 단위 |
|---|---|---|
| NH4Cl | 0.33 | g |
| KH2PO4 | 0.33 | g |
| KCl | 0.33 | g |
| MgCl2·6 H2O | 0.33 | g |
| CaCl2·2 H2O | 0.33 | g |
| Trace element SL-10 (see medium 497) | 1.0 | ml |
| Yeast extract | 0.50 | g |
| Resazurin (0.1% w/v) | 0.50 | ml |
| Vitamin solution (see medium 334) | 10.0 | ml |
| NaHCO3 | 1.5 | g |
| Cellobiose or Soluble starch | 5.0 | g |
| Na2S x 9 H2O | 0.5 | g |
| Distilled water | 1000 | ml |
| Dissolve ingredients (except vitamins, bicarbonate, cellobiose and sulfide), boil medium for 1 min., then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. If starch is used as substrate it should be dissolved separately in a small amount of distilled water and added to the medium before autoclaving. Dispense medium under same gas atmosphere in culture vessels and autoclave. Prior to inoculation add vitamins, cellobiose and sulfide from sterile anoxic stock solutions prepared under N2 and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 (cellobiose should be sterilized by filtration). The pH of the completed medium should be at 7.1 |
** ph : -
** memo : DSM 516 medium
** 추가조치 사항 : -
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스