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Distribution
조성 | 용량 | 단위 |
---|---|---|
Na2CO3 | 10.0 | g |
NaHCO3 | 15.0 | g |
NaCl | 10.0 | g |
K2HPO4 | 0.2 | g |
NH4Cl | 1.0 | g |
KCl | 0.2 | g |
Na2S · 9 H2O | 1.0 | g |
Yeast extract | 0.5 | g |
Sucrose | 5.0 | g |
Vitamin solution (see medium 334) | 10.0 | ml |
Trace element solution (see medium 334) | 1.0 | ml |
Distilled water | 1000 | ml |
Prepare the medium under N2, Omitting NaHCO3, Na2S, sucrose and vitamin solution. Boil the medium and cool under nireogen, add the NaHCO3 and adjust the pH to 9.7 with 6N NaOH(about 15ml). Sterilize by autoclaving and add the Na2S(neutralized), vitamins and sucrose from sterile stock solutions. |
** ph : -
** memo : -
** 추가조치 사항 : -