KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
| 조성 | 용량 | 단위 | ||||||
|---|---|---|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | ||||||
| MgSO4·7H2O | 0.5 | g | ||||||
| NaCl | 0.4 | g | ||||||
| NH4Cl | 0.68 | g | ||||||
| CaCl2·2H2O | 0.25 | g | ||||||
| Sodium pyruvate | 0.5 | g | ||||||
| Succinate | 0.5 | g | ||||||
| Acetate | 0.5 | g | ||||||
| Yeast extract | 0.5 | g | ||||||
| Ferric Citrate (0.1% [w/v]stock) (see medium 935) | 5.0 | ml | ||||||
| Micronutrient solution SL7 (see medium 935) | 1.0 | ml | ||||||
| Vitamin B12 (from 2mg/100ml) (see medium 935) | 1.0 | ml | ||||||
| NaHCO3 solution (10% w/v) (see below) | 15.0 | ml | ||||||
|
||||||||
| Na2S·9H2O(1M) | 1.0 | ml | ||||||
|
||||||||
| Final pH 7.0 | ||||||||
| Distilled water | 1.0 | L | ||||||
| Mix ingredients except for vitamin B12, NaHCO3 and Na2S·9H2O, and autoclave under a N2 atmosphere. Separately autoclave Na2S·9H2O solution under a N2 atmosphere. Sterilize vitamin B12 and NaHCO3 solutions by filtration. Prior to inoculation, aseptically and anaerobically add them. | ||||||||
** ph : -
** memo : -
** 추가조치 사항 : -