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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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자원 통합 검색
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 1438 : GEOBACTER MEDIUM
| 조성 | 용량 | 단위 |
|---|---|---|
| NH4Cl | 1.5 | g |
| NaH2PO4 | 0.6 | g |
| KCl | 0.1 | g |
| NaHCO3 | 2.5 | g |
| Sodium acetate | 0.82 | g |
| Sodium fumarate (filter sterilized) | 8 | g |
| Vitamine solution (see medium 1335) | 10.0 | ml |
| Trace element solution (see medium 918) | 10.0 | ml |
| D.W | 976 | ml |
| 0.025% Resazurin | 4 | ml |
** ph : -
** memo : 10 mM sodium acetate (electron donor), 20 mM ferric citrate (electron acceptor)
** 추가조치 사항 : Dissolve ingredients (except bicarbonate, fumarate and vitamins), sparge medium with
80% N2 and 20% CO2 gas mixture for 30 ? 45 min to make it anoxic. Add bicarbonate
and equilibrate the medium with 80% N2 and 20% CO2 gas mixture to reach a pH of 6.8,
then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes
or serum vials and autoclave. After autoclaving add fumarate and vitamins from anoxic
stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration.
Check medium pH and adjust to 6.8, if necessar
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스