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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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자원 통합 검색
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 866 : CALDICELLULOSIRUPTOR MEDIUM
| 조성 | 용량 | 단위 |
|---|---|---|
| NH4Cl | 0.90 | g |
| NaCl | 0.90 | g |
| MgCl2·6H2O | 0.40 | g |
| KH2PO4 | 0.75 | g |
| K2HPO4 | 1.50 | g |
| Trypticase peptone (BD BBL) | 2.00 | g |
| Yeast extract (OXOID) | 1.00 | g |
| Trace element solution SL-10 (see medium 1417) | 1.00 | ml |
| FeCl3·6H2O (0.1% w/v in 0.2 N HCl) | 2.50 | ml |
| L-Cysteine HCl x H2O | 0.75 | g |
| Sodium resazurin (0.1% w/v) | 0.50 | ml |
| Distilled water | 1000.00 | ml |
| Cellobiose | 1.0 | g |
** ph : -
** memo : Cellobiose 1.00 g or Cellulose 1.00 g
** 추가조치 사항 : Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for
30 - 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Distribute medium
under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2
gas atmosphere and sterilized by filtration. Adjust pH of the complete medium to 7.2, if
necessary.
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스