Korean Collection for Type Cultures

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Distribution

분양

일반자원검색

KCTC Media No. 1018 : FWM-MEDIUM

조성

용량

단위

Solution A:

조성	용량	단위
CaCl₂·2H₂O	0.15	g
Distilled water	10.0	ml
KH₂PO₄	0.20	g
NH₄Cl	0.25	g
NaCl	1.00	g
MgCl₂ · 6 H₂O	0.40	g
KCl	0.50	g
Resazurin (0.1% W/V)	0.50	ml
Distilled water	940.00	ml

Solution B: Trace element solution SL-10 (see medium 497)

1.00

Solution C: Vitamins solution (see below)

1.00

조성	용량	단위
Vitamin B₁₂	100	mg
p-Aminobenzoic acid	80.00	mg
D(+)-Biotin	20.00	mg
Nicotinic acid	200.00	mg
Calcium pantothenate	100.00	mg
Pyridoxine hydrochloride	300.00	mg
Thiamine-HCl x 2 H2O	200.00	mg
Distilled water	1000.00	ml

Solution D: Selenite-tungstate solution (see below)

1.00

조성	용량	단위
NaOH	0.5	g
Na₂SeO₃ x 5 H₂O	3.0	mg
Na₂WO₄ x 2 H₂O	4.0	mg
Distilled water	1000	ml

Solution E:

조성	용량	단위
Distilled water	30.0	ml
Na₂CO₃	1.5	g

Solution F: ··Substrate solution (see below)··

solution G:

조성	용량	단위
Na₂S·9H₂O	0.3	g
Distilled water	10.0	ml

Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, D and G are autoclaved separately under 100% N2 gas. Solutions C and F are prepared under 100% N2 gas and sterilized by filtration. Solution E is autoclaved under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4, if necessary.

** ph : -

** memo : -

** 추가조치 사항 : Solution F:
**Substrate solution**
For KCTC15201
Use 2.00 g/l of xylan or xylose as substrate.
For KCTC15912
Use 2.00 g/l yeast extract and 5.00 g/l D-glucose as substrates added from anoxic stock solutions sterilized by filtration.
For KCTC4119
Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and
adjust pH of the medium to 6.5 - 7.0. After inoculation add 10.00 ml/l of a sterile anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v).

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