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미생물클러스터
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MICROBIOTA
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회원가입
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ENG
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Korean Collection for Type Cultures
KCTC에서는 일반자원 분양과 특허자원 분양 서비스를 제공 합니다.
Distribution
분양
일반자원검색
KCTC Media No. 1095 : Methanomethylovorans medium for KCTC4119
| 조성 | 용량 | 단위 |
|---|---|---|
| KCl | 0.5 | g |
| KH2PO4 | 0.2 | g |
| NaCl | 1.0 | g |
| NH4Cl | 0.5 | g |
| CaCl2·2H2O | 0.1 | g |
| MgCl26H2O | 0.4 | g |
| D·W | 1.0 | L |
| Vitamin solution (see medium 1335 ) | 1.0 | ml |
| Trace element SL-10 (see medium 497) | 1.0 | ml |
| selenite tungstate solution (see medium 1018) | 1.0 | ml |
| 30%Trimethylamine-HCl soiution | 10.0 | ml |
| 8% NaHCO3 solution | 20.0 | ml |
| 5% Na2S·9H2O solution | 8 | ml |
| Resazurin | 1.0 | mg |
** ph : 6.5
** memo : -
** 추가조치 사항 : Mix ingredients except trimethylamine, vitamin solution, NaHCO3 and Na2S·9H2O, and autoclave under a N2/CO2 (80/20) atmosphere. Separately autoclave Na2S·9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add filter-sterile trimethylamine as 30% solution, NaHCO3 as 8% solution and vitamin solution to the medium. Prior to inoculation, add the sterile Na2S·9H2O solution. Pressurize the inoculated vessels to 150 kPa with N2/CO2.
담당자
기탁/분양서류
DNA분양서비스
동결건조서비스
STR분석서비스