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Distribution
조성 | 용량 | 단위 |
---|---|---|
Baker's Yeast | 5.0 | g |
CaCl2·2H2O | 1.0 | g |
Vitamin B12 | 0.50 | mg |
Agar | 15.0 | g |
Distilled water | 1.0 | L |
** ph : 7.0-7.5
** memo : -
** 추가조치 사항 : Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved
stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and
autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and
cooling to 50?C (use pH-indicator paper).